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1.
Journal of Sun Yat-sen University(Medical Sciences) ; (6): 560-564, 2019.
Article in Chinese | WPRIM | ID: wpr-817719

ABSTRACT

@#【Background】 The aim of this prospective randomized study was to evaluate the feasibility of subtotal thyroidectomy leaving a unilateral remnant based on the upper pole. 【Methods】 Patients who underwent the subtotal thyroidectomy and isthmusectomy leaving either a unilateral remnant based on the upper pole(GroupⅠ ,59 patients)or with the bilateral dorsal thyroid tissue remained(Group Ⅱ,54 patients)were prospectively compared in operation time, blood loss,recurrence,and postoperative complications.【Results】The operation time remained similar between the two groups,but the blood loss,the reoperation time,recurrent laryngeal nerve injury,and recurrence in GroupⅠwere much less than those in Group Ⅱ. In addition ,no postoperative hemorrhage occurred in GroupⅠ. Three patients (5.56%) underwent postoperative hemorrhage in Group Ⅱ. Two patients(3.39%)in GroupⅠand 4 patients(7.40%)in Group Ⅱ experienced transient hypocalcemia.【Conclusion】In terms of blood loss,reoperation time,postoperative complication, and recurrence ,subtotal thyroidectomy with recurrent laryngeal nerves identification and the unilateral superior pole remnant of the gland provides a better outcome than subtotal thyroidectomy with bilateral dorsal thyroid tissue remnant.

2.
Chongqing Medicine ; (36): 2148-2152, 2018.
Article in Chinese | WPRIM | ID: wpr-692071

ABSTRACT

Objective To explore the effect of down-regulation of structural maintenance of chromosome 4 (SMC4) on proliferation,migration and invasion capability of human breast cancer cell line MDA-MB-231 and its possible mechanism.Methods The expressions of SMC4 in breast cancer tissues and the corresponding adjacent tissues from 20 patients with breast cancer were detected by qPCR.The expressions of SMC4 in human mammary epithelial cell line (MCF10A) and breast cancer cell lines (MDA-MB-231,T47D,SK-BR-3,MCF7 and MDA-MB-468) were detected by qPCR and western blot.After down-regulated the expression of SMC4 in MDA-MB-231 by small interfering RNA (siRNA),qPCR and western blot were used to determine the effect of transfection,CCK8 and clone formation assay were used to detect the proliferation and clonogenicity,Transwell chamber assay was used to detect the migration and invasion,and the possible pathway associated proteins were detected by western blot.Results The expression level of SMC4 in breast cancer tissues was higher than that in corresponding adjacent tissues (P<0.05).The expression levels of SMC4 in breast cancer cell lines (MDA-MB-231,T47D,SK-BR-3,MCF7 and MDA-MB-468) were higher than that in MCF10A (P<0.05).After successfully down-regulated SMC4 expression by siRNA,the proliferation,migration and invasion capability of MDA-MB-231 were significantly decreased (P<0.05),and the expressions of pAKT and p-PI3K were significantly decreased (P<0.05),whereas theexpressions of AKT and PI3K were not significantly affected.Conclusion Down-regulating the expression of SMCA can inhibit proliferation,migration and invasion capability of MDA-MP-231,which may be related to the activation of PI3K/AKT signaling pathway.

3.
Journal of Southern Medical University ; (12): 56-61, 2015.
Article in Chinese | WPRIM | ID: wpr-239247

ABSTRACT

<p><b>OBJECTIVE</b>To establish a NOD/SCID mouse model with human immune reconstitution and observe its immune response to human triple-negative breast cancer xenograft.</p><p><b>METHODS</b>Twenty-four NOD/SCID mice without immune leakage were subjected to cyclophosphamide (CTX) treatment 3 days prior to immune reconstitution with human peripheral blood mononuclear cell (PBMC) injection and subcutaneous transplantation of human triple-negative breast cancer MDA-MB-231 cells, CTX treatment and PBMC injection without tumor cell transplantation, MDA-MB-231 cell transplantation only, or no treatments. The tumor growth and immune responses of the mice were observed at regular intervals.</p><p><b>RESULTS</b>Compared with the tumor-bearing mice, the tumor-bearing mice with immune reconstitution showed prolonged incubation period of tumor formation, slower tumor growth rate and increased survival rate. Human IgG and CD3(+) T cells were detected in the peripheral blood of the mice 1 week after human PBMC injection. The percentage of CD3(+) T cells in the spleen cells was 55.3% at 9 weeks in tumor-bearing mice with immune reconstitution and 52.7% in tumor-bearing mice without immune reconstitution. The spleen index of the tumor-bearing mice with immune reconstitution was much higher than that in mice with only immune reconstitution and the control mice (9.64 vs 3.82∓0.31 and 1.51∓0.14 mg/g).</p><p><b>CONCLUSION</b>A stable NOD/SCID mouse model with immune reconstitution has been established successfully, which shows immune responses to triple-negative breast cancer xenografts and allows studies of immunological therapy study of triple-negative breast cancer.</p>


Subject(s)
Animals , Humans , Mice , Disease Models, Animal , Leukocytes, Mononuclear , Mice, Inbred NOD , Mice, SCID , Neoplasm Transplantation , Spleen , Allergy and Immunology , Triple Negative Breast Neoplasms , Allergy and Immunology
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